Is Circovirus DNA Infectious?
Saturday, March 27, 2010 at 06:45 AM
The US Food and Drug
Administration does not want Rotarix, the rotavirus vaccine, to be used
it contains porcine circovirus 1 DNA. If complete copies of the
circovirus genome were present, would they constitute a potential threat to
recipients? Put another way, is circovirus DNA infectious?
Here is the information you need to answer this question.
- The circovirus genome is a circular, single stranded DNA molecule
- To infect a cell, the two viral proteins encoded in the DNA must be
- To produce proteins, mRNA must be synthesized from the viral DNA.
- Single-stranded DNA cannot be copied into mRNA; the DNA must be
- The circovirus particle consists of a protein shell surrounding the viral
DNA. There are no other components in the virion.
- During infection of cells by circoviruses, the particles enter the nucleus
where the viral DNA is released
- If naked DNA is added to cells, a good fraction ends up in the
Knowing these facts, can you determine whether introduction of circovirus
DNA into cells would lead to viral replication?
This article originally appeared on virology blog.